To understand the functional operation of the brain, it is necessary to use novel imaging techniques such as 3D two-photon microscopy, which allows real-time measurement and activation of neuronal somas and extensions, enabling the inspection of network mechanisms. Our proprietary 3D AO scanning technology has significantly increased measurement speed and signal-to-noise ratio efficiency. Additionally, we have developed an online, real-time motion correction method for eliminating movement that occurs during in vivo experiments. As a result, it is now possible to rapidly measure pyramidal cell and interneuron activity in 3D in moving, behaving animals using both calcium sensors and the recently introduced voltage sensors.
Through our measurements, we are discovering previously unknown signaling systems in the brain related to reward and punishment that influence learning processes across the entire neocortex and hippocampus. In this laboratory, we focus mainly on developing and improving photostimulation methods. In our laboratories at BrainVisionCenter, our primary research facility established in collaboration with Botond Roska, we are making progress with ongoing human retinal measurements We currently have more than 40 international patents.
