Tantárgy adatlapja
During the lectures, the students get familiar with the following concepts and examples: Definition of recombinant DNA, genomic and cDNA libraries. Molecular hybridization techniques. The Human Genome Project. Differences between individual genomes. Gene identification and genome annotation. Polymerase chain reaction and targeted mutagenesis. Gene expression platforms. Cloning into bacteria. Insulin, the first human recombinant medicine. Trasgenic animals and animal cloning. Use of recombinant DNA techniques in the pharmaceutical industry. Human gene therapy. Bioinformatics in recombinant DNA technology.
In the practicals, the sudents perform the following experiments: Preparation of genomic DNA and genotyping by PCR-RFLP, PCR-ASA and real-time PCR; Isolation and purification of plasmid DNA, restriction mapping; PCR amplification of recombinant DNA fragments and subsequent agarose gel electrophoresis; Protein expression using bacterial cells and in vitro translation systems.
