Tantárgy adatlapja
Description of the subject:
1.Introduction to headfixed mouse behavior experiments
2.Headfixed or freely moving? A comparison between paradigms.
3.How to design a mouse headfixed setup? – an overview
4.Mechanical components of behavior experimental setups
5.Electric components of behavior experimental setups
6.Firmware design of headfixed behavior controllers
7.Firmware implementation guidelines behavior setup controllers
8.Stimuli delivery methods for headfixed behavioral paradigms
9.Designing water reward systems for headfixed experiments
10.Pavlovian conditioning in headfixed experiments
11.Ethical considerations and welfare guidelines for headfixed animal experiments
12.Advanced experimental designs: combining headfixed behavior with electrophysiology or optogenetics
Selected literature:
[1]R. Aoki, R. Aoki, T. Tsubota, Y. Goya, and A. Benucci, “An automated platform for high-throughput mouse behavior and physiology with voluntary head-fixation.,” Nat. Commun., 2017, doi: 10.1038/s41467-017-01371-0.
[2]Z. V Guo et al., “Procedures for behavioral experiments in head-fixed mice.,” PLoS One, 2014, doi: 10.1371/journal.pone.0088678.
[3]C. Barkus et al., “Refinements to rodent head fixation and fluid/food control for neuroscience,” J. Neurosci. Methods, 2022, doi: 10.1016/j.jneumeth.2022.109705.
[4]Z. Han, X. Zhang, J. Zhu, Y. Chen, and C. T. Li, “High-Throughput Automatic Training System for Odor-Based Learned Behaviors in Head-Fixed Mice.,” Front. Neural Circuits, 2018, doi: 10.3389/fncir.2018.00015.
[5]K. Juczewski, J. A. Koussa, A. J. Kesner, A. J. Kesner, J. O. Lee, and D. M. Lovinger, “Stress and behavioral correlates in the head-fixed method,” bioRxiv, 2020, doi: 10.1101/2020.02.24.963371.
Required skills:
headfixed behavior experiment, mechanical design, electrical design, C/C++ programming skills
tml>
